ANTI-MOUSE CD19 (EBIO1D3) BIOTIN 1 M

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ANTI-MOUSE CD19 (EBIO1D3) BIOTIN 1 M

货号:13-0193-86

规格:BIOTIN 1 M

价格:4772

产品类型:流式抗体

品牌:eBioscience

抗原:CD19

物种:小鼠

宿主:大鼠

抗体亚型:IgG

克隆号:eBio1D3

荧光染料:Biotin

抗体类型:单抗同型对照:IgG2a, kappa
免疫原性:应用领域:Flow;MISC;FN;IHC
推荐用法:0.5 µg/test(flow)

Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD19 Antibody (13-0193-82)

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control Biotin (Product # 13-4321-82) (left) or 0.125 µg of Anti-Mouse CD19 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

推荐产品:
SuperSignal™ West Pico PLUSFIX & PERM® Cell Permeabilization KitELISA Wash Buffer (30X)
参考文献:
1.Circulation researchAMP-Activated Protein Kinase α2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1α and a Network of Proteins Affecting Metabolism and Apoptosis."13-0193 was used in Flow cytometry/Cell sorting to elucidate the role of the AMPKa2 catalytic subunit in vascular repair following ischemia."Authors Abdel Malik R,Zippel N,Frömel T,Heidler J,Zukunft S,Walzog B,Ansari N,Pampaloni F,Wingert S,Rieger MA,Wittig I,Fisslthaler B,Fleming I2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.3. Schopperle, W.M., and DeWolf, W.C. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730.4. Chin, A.C., Fong, W.J., Goh, L.T., Philp, R., Oh, S.K., and Choo, A.B. (2007) Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol. 130: 320-328.5. Badcock, G., Pigott, C., Goepel, J., and Andrews, P.W. (1999) The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 59: 4715-4719.6. Andrews, P.W., Banting, G., Damjanov, I., Arnaud, D., and Avner, P. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361.

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