anti-mouse CD19 (eBio1D3) eFluor 450

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anti-mouse CD19 (eBio1D3) eFluor 450

货号:48-0193-80,48-0193-82

规格:25μg,100μg

价格:1172,2073

产品类型:流式抗体

品牌:eBioscience

抗原:CD19

物种:小鼠

宿主:大鼠

抗体亚型:IgG

克隆号:eBio1D3

荧光染料:eFluor 450

抗体类型:单抗同型对照:IgG2a, kappa
免疫原性:应用领域:Flow;FN;IHC
推荐用法:0.5 µg/test(flow)

Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD19 Antibody (48-0193-82)

Staining of C57BL/6 splenocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and 0.25 µg of Rat IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse CD19 eFluor® 450 (right). Total viable cells were used for analysis.

推荐产品:
Flow Cytometry Staining Buffer, eBioscienceUltracomp eBeads microspheresLIVE/DEAD® Fixable Cell Stains
参考文献:
1.The Journal of experimental medicineEros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity."48-0193 was used in Flow cytometry/Cell sorting to describe the key role of the previously uncharacterised protein Eros in host defence."Authors Thomas DC,Clare S,Sowerby JM,Pardo M,Juss JK,Goulding DA,van der Weyden L,Storisteanu D,Prakash A,Espéli M,Flint S,Lee JC,Hoenderdos K,Kane L,Harcourt K,Mukhopadhyay S,Umrania Y,Antrobus R,Nathan JA,Adams DJ,Bateman A,Choudhary JS,Lyons PA,Condliffe AM,Chilvers ER,Dougan G,Smith KG2.PloS oneDifferential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase."48-0193 was used in Flow cytometry/Cell sorting to demonstrate a complex regulation pattern of C5aR1 in the airways, lung tissue and lymph nodes of mice."Authors Ender F,Wiese AV,Schmudde I,Sun J,Vollbrandt T,König P,Laumonnier Y,Köhl J3.The Journal of experimental medicineDC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo."48-0193 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice."Authors Lehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D4.Journal of orthopaedic research : official publication of the Orthopaedic Research SocietyDiminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity."48019382 was used in flow cytometry to infer a RANKL-dependent osteoclastogenesis after debridement of osteomyelitis coinciding with elevated B cells and simultaneously decreased osteogenesis"Authors Wagner JM,Jaurich H,Wallner C,Abraham S,Becerikli M,Dadras M,Harati K,Duhan V,Khairnar V,Lehnhardt M,Behr B5.Nature communicationsIL-21 restricts T follicular regulatory T cell proliferation through Bcl-6 mediated inhibiion of responsiveness to IL-2."48-0193 was used in Flow cytometry/Cell sorting to identify a mechanism by which IL-21 reinforces humoural immunity by influencing the phenotype of T follicular cells to limit their expansion."Authors Jandl C,Liu SM,Cañete PF,Warren J,Hughes WE,Vogelzang A,Webster K,Craig ME,Uzel G,Dent A,Stepensky P,Keller B,Warnatz K,Sprent J,King C

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