anti-mouse CD19 (eBio1D3) Biotin
货号：13-0193-85
规格：500 μg
价格：2729
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：eBio1D3
荧光染料：Biotin
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 应用领域： | Flow；MISC；FN;IHC |
| 推荐用法： | 0.25 µg/test（flow) |
| Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD19 Antibody (13-0193-82) Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control Biotin (Product # 13-4321-82) (left) or 0.125 µg of Anti-Mouse CD19 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis. |
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