CD44 Monoclonal Antibody (IM7), Alexa Fluor 700, eBioscience™

货号： 56-0441-80, 56-0441-82

规格：25 µg,100 µg

价格：1038,2583

产品类型：流式抗体

品牌：eBioscience

抗原：CD44

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：IM7

荧光染料：Alexa Fluor 700

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, Alexa Fluor 700, eBioscience™
免疫原性：		用法：	0.06 µg/test(Flow）

Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: This IM7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD44 Antibody (56-0441-80) in FlowStaining of BALB/c splenocytes with 0.03 µg of Rat IgG2b K Isotype ControlAlexa Fluor® 700 (Product # 56-4031-80) (open histogram) or 0.03 µg of Anti-Human/MouseCD44 Alexa Fluor® 700 (filled histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.TheranosticsGlutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.2.Journal of immunology (Baltimore, Md. : 1950)The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.3.Molecular medicine reportsBreast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics.4.PloS oneMulti-lineage differentiation of human umbilical cord Wharton's Jelly Mesenchymal Stromal Cells mediates changes in the expression profile of stemness markers....5.Stem cells (Dayton, Ohio)The CD44+ ALDH+ population of human keratinocytes is enriched for epidermal stem cells with long-term repopulating ability.

技术参数

产品应用 Flow；IF；ICC；IHC

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