| Description: The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.Applications Reported: This 4H11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H11 (APG) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.PloS onePDL1 Expression on Plasma and Dendritic Cells in Myeloma Bone Marrow Suggests Benefit of Targeted anti PD1-PDL1 Therapy."12-0349 was used in Flow cytometry/Cell sorting to investigate whether anti PDL1 or PD-1 treatment targeting the immune system could be used against multiple myeloma."AuthorsSponaas AM,Moharrami NN,Feyzi E,Standal T,Holth Rustad E,Waage A,Sundan A2.Stem cells translational medicineEnforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells."12-0349 was used in Flow cytometry/Cell sorting to investigate whether activation of exogenous HOXB4 promotes the production of more mature, definitive red blood cells."AuthorsJackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM3. The Journal of biological chemistrySuppression of homeobox transcription factor VentX promotes expansion of human hematopoietic stem/multipotent progenitor cells."12-0349 was used in Flow cytometry/Cell sorting to study the mechanisms that regulate the expansion of human hematopoietic stem/multipotent progenitor cells, showing that VentX regulates critical cell cycle regulators and Wnt downstream genes previously implicated in HSC/MPP proliferation and expansion."AuthorsGao H,Wu X,Sun Y,Zhou S,Silberstein LE,Zhu Z4NeoplasmaGrowth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines.AuthorsElknerová K,Lacinová Z,Soucek J,Marinov I,Stöckbauer P |
QQ:1749072012