Acetyl-alpha Tubulin (Lys40) Monoclonal Antibody (6-11B-1)/乙酰化α-Tubulin内参抗体

货号：32-2700

规格：100 µg

价格：4786

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：其它

点击Invitrogen内参抗体集，查看更多内参抗体

抗体类型:	单抗	同型对照：	IgG2b
免疫原性：	Acetylated alpha-tubulin from the axoneme of sea urchin sperm flagella.	用法：	1.0 -3.0µg/mL(ICC）;1.0 -3.0µg/mL(IF）;1.0 -3.0µg/mL(WB)

Product Specific InformationThis antibody reacts with the ~ 55 kDa acetylated form of alpha-tubulin. This antibody reacts with human, mouse, and rat. Reactivity is confirmed with NIH3T3 and HeLa cells.Background/Target InformationAlpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Acetyl-alpha Tubulin (Lys40) Antibody (32-2700) in IFImmunofluorescent analysis of Alpha-Tubulin (Acetylated) was done on 70% confluent log phase HeLa cells treated with 5mM sodium butyrate for 24 h. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Alpha-Tubulin (Acetylated) Recombinant Mouse Monoclonal Antibody (Product # 32-2700) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing Alpha-Tubulin (Acetylated) in cytoplasm and panel e is a no primary antibody control. The images were captured at 20X magnification. Acetyl-alpha Tubulin (Lys40) Antibody (32-2700) in FlowHuman naïve CD4 T cells were activated with anti-CD3/CD28 beads (1:1) (Product # 11132D) for 3 days. Cells were stained with Acetyl-alpha Tubulin (Lys40) Monoclonal Antibody (6-11B-1) (Product # 32-2700) at the concentration of 1:400 for 2 hours on ice, followed by incubation with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Product # A-11032) at the concentration of 1:200 for 2 hours on ice. FMO: Fluorescence Minus One. Data courtesy of the antibody data exchange program.

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参考文献：

1.Scientific reportsIdentification of Elongated Primary Cilia with Impaired Mechanotransduction in Idiopathic Scoliosis Patients."322700 was used in immunocytochemistry to assess the phenotype of bone-derived primary cilia from patients with idiopathic scoliosis"AuthorsOliazadeh N,Gorman KF,Eveleigh R,Bourque G,Moreau A2.Developmental cellThe Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis."32-2700 was used in immunocytochemistry to study Shh signaling during oncogenesis and hindbrain development due to Eya1 phosphate promotion"AuthorsEisner A,Pazyra-Murphy MF,Durresi E,Zhou P,Zhao X,Chadwick EC,Xu PX,Hillman RT,Scott MP,Greenberg ME,Segal RA3.Human molecular geneticsGM130 gain-of-function induces cell pathology in a model of lysosomal storage disease."32-2700 was used in immunocytochemistry to investigate the role of GM30 during lysosomal biogenesis."AuthorsRoy E,Bruyère J,Flamant P,Bigou S,Ausseil J,Vitry S,Heard JM4.Nature medicineImpaired Wnt-beta-catenin signaling disrupts adult renal homeostasis and leads to cystic kidney ciliopathy."32-2700 was used in immunocytochemistry to explore the role of canonical Wnt activity in adult mammalian kidney homeostasis"AuthorsLancaster MA,Louie CM,Silhavy JL,Sintasath L,Decambre M,Nigam SK,Willert K,Gleeson JG5.Scientific reportsThe molecular motor Myosin Va interacts with the cilia-centrosomal protein RPGRIP1L."322700 was used in proximity ligation assay to analyze proteins interacting with myosin Va at the centrosome"AuthorsAssis LH,Silva-Junior RM,Dolce LG,Alborghetti MR,Honorato RV,Nascimento AF,Melo-Hanchuk TD,Trindade DM,Tonoli CC,Santos CT,Oliveira PS,Larson RE,Kobarg J,Espreafico EM,Giuseppe PO,Murakami MT

技术参数

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