Pierce™ Protease Inhibitor Tablets, EDTA-free/ 蛋白酶抑制剂，不含EDTA
货号：A32965
规格：20 tablets
价格：3255
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| Thermo Scientific Pierce Protease Inhibitor Tablets, EDTA-free, contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.Pierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A. Stable for storage at 4°C for up to 12 months, each tablet is sufficient for 50 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases reside in cellular compartments, but upon cell lysis the proteases are released, resulting in the degradation of valuable proteins. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts.To use Pierce Protease Inhibitor Tablets, simply dissolve 1 tablet in 50 mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Pierce Protease Inhibitor Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce Phosphatase Inhibitor Mini Tablets, can be added along with the protease inhibitor tablets. |
| 优点： |
| - Improved formulation—tablets dissolve quickly to form a clear solution;- Economical—more cost effective than other commercially available formulations for equivalent volumes;- Convenient—ready-to-use, fully disclosed, broad-spectrum formulations;- Compatible—use directly with Pierce BCA assays;- EDTA-free—formulated without EDTA, a metalloproteinase inhibitor (only for EDTA-free formulations)。 |
| 数据： |
图 1 Comparison of commercially-available protease inhibitor tabletsPancreatic extract (100 µL; 0.5 µg/µL) was incubated with fluorescent protease-cleavable substrates for trypsin, cysteine, aminopeptidase, and metalloprotease/cathepsins in the presence of commercially-available protease inhibitors with and without EDTA. Reactions were incubated for 1 hour at 37ºC and fluorescence was measured. The percent protease inhibition is shown for each protease inhibitor formulation. |
| 相关产品： |
| ▪NE-PER™ Nuclear and Cytoplasmic Extraction Reagents（货号#78833/78835）▪Mem-PER™ Plus Membrane Protein Extraction Kit（货号#89842）▪M-PER™ Mammalian Protein Extraction Reagent（货号#78501/78505）▪Halt™ Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)（货号#78442/78440/78444/78446）▪ Pierce Protease Inhibitor Tablets（货号#A32963）▪Pierce™ Protease Inhibitor Mini Tablets, EDTA-fre（货号#32955）▪Pierce™ Phosphatase Inhibitor Mini Tablets（货号#32957）▪Pierce Protease and Phosphatase Inhibitor Mini Tablets（货号#32959） |
技术参数 产品优点 1. Improved formulation—tablets dissolve quickly to form a clear solution；
2. Economical—more cost effective than other commercially available formulations for equivalent volumes；
3. Convenient—ready-to-use, fully disclosed, broad-spectrum formulations。
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