Anti-Human CD19 (HIB19) functional grade
货号:16-0199-85
规格:500 μg
价格:4153
产品类型:流式抗体
品牌:eBioscience
抗原:CD19
物种:人
宿主:小鼠
抗体亚型:IgG
克隆号:HIB19
荧光染料:其它
抗体类型: | 单抗 | 同型对照: | IgG1, kappa |
免疫原性: | | 用法: | 1µg/test(Flow) |
The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.Applications Reported: The HIB19 antibody has been reported for use in functional assays and flow cytometric analysis. (Fluorochrome-conjugated HIB19 is recommended for use in flow cytometry.).Applications Tested: This HIB19 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC. |
数据 |
CD19 Antibody (16-0199-85) in Flow Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (left) or PE (right). Autofluorescence is indicated by open histogram. Cells in the lymphocyte gate were used for analysis. |
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ANTI-HUMAN CD19 (HIB19) FITC(货号#11-0199)ANTI-HUMAN CD19 (SJ25C1) PE(货号#12-0198)ANTI-HUMAN CD19 (HIB19) PE(货号#12-0199)ANTI-HUMAN CD19 (HIB19) PE (货号#12-0199-80) |
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