ANTI-HUMAN CD19 (HIB19) PE-CYANINE 5
货号：15-0199-41,15-0199-42
规格：25TESTS,100 TESTS
价格：0,2629
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：HIB19
荧光染料：PE-Cy5
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 5 µL (0.25µg)/test（Flow） |
| Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD19 Antibody (16-0199-85) in Flow Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (left) or PE (right). Autofluorescence is indicated by open histogram. Cells in the lymphocyte gate were used for analysis. |
| 推荐产品： |
| ANTI-HUMAN CD19 (HIB19) FITC（货号#11-0199）ANTI-HUMAN CD19 (SJ25C1) PE（货号#12-0198）ANTI-HUMAN CD19 (HIB19) PE（货号#12-0199）ANTI-HUMAN CD19 (HIB19) PE (货号#12-0199-80） |
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