图1.Data for CFSET-cell receptor transgenic, OVA-reactive OT-I cells undergo several rounds of cell division in response to OVA, but not PBS,in vivo. Lymph node cells from OT-I mice were labeled with 1 µM CFSE and adoptively transferred into C57Bl/6 mice. Mice were then immunized with OVA or PBS. Three days later, splenocytes were stained with Anti-Mouse/Rat CD90.1 (Thy-1.1) PerCP-Cyanine5.5 (cat. 45-0900) and Anti-Mouse CD8a eFluor™ 450 (cat. 48-0081) to gate on the OT-I cells (left). Cells in the OT-I gate (CD8+Thy1.1+) were analyzed for cell division (right), measured as discrete peaks of decreasing fluorescence of CFSE in OVA-immunized mice (purple histogram), or undivided cells with a single, bright CFSE peak in PBS-immunized mice (blue histogram).