CD3 Monoclonal Antibody (HIT3a), FITC, eBioscience™
货号：11-0039-42
规格：100 tests
价格：628
产品类型：流式抗体
品牌：eBioscience
物种：人
宿主：小鼠
抗体亚型：IgG2a, kappa
克隆号：HIT3a
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| 浓度： | 5 µL/Test | 用法： | 5uL（1 µg）/test(Flow） |
| 产品详细信息Description: The HIT3a monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the ? chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIT3a antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency. |
| 数据 |
CD3 Antibody (11-0039-41) in Flow Normal human peripheral blood cells were stained with Mouse IgG2a kappa Isotype Control, FITC (Product # 11-4724-82) (blue histogram) or CD3 Monoclonal Antibody, FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.CD3 Antibody (11-0039-41)Cell research 2017 -Figure 2 Naive CD4 + T cell abundance within breast tumors is associated with increased numbers of Tregs and poor patient prognosis. (A) IHC staining of CD3 (red) and CD45RA (brown) in a representative breast cancer sample. Naive T cells were defined as CD3 + CD45 + cells. Scale bar, 50 mum. (B) Correlation of TI naive CD4 + T cell numbers and TI Treg numbers in breast cancer samples ( n = 626, Pearson correlation coefficient R and P -value are shown). (C) Representative immunofluorescent staining for naive T cells (CD3 (red), CD45RA (green) and CD4 (purple); upper panels) or Tregs (Foxp3 (green) and CD4 (purple); lower panels) in serial sections from a human breast cancer sample. Arrows indicate CD3 + CD4 + CD45RA + naive CD4 + T cells (upper) and CD4 + Foxp3 + Tregs (lower). Scale bar, 50 mum. The localization of naive CD4 T cells and Tregs relative to the perivascular space or tumor parenchyma for 626 tumor samples is provided in Supplementary information, Figure S3C . (D) Representative immunofluorescent staining of CD3 (red), CD45RA (green), CD4 (purple) and DAPI (blue) in breast cancer samples with high (upper panel) or low (lower panel) number of naive CD4 + T cells, which are indicated by arrows. Scale bar, 50 mum. (E) Kaplan-Meier survival curve of breast cancer patients with low and high numbers of TI naive CD4 + T cells. BV, blood vessel; TN, tumor nest. |
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| 参考文献： |
| 1.OncoTargets and therapy The elevated glutaminolysis of bladder cancer and T cells in a simulated tumor microenvironment contributes to the up-regulation of PD-L1 expression by interferon-γ.2.Acta Cardiologica Sinica The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.3.Cell research Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer.4.The Journal of clinical investigation Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.5.PloS one Accumulation of self-reactive naïve and memory B cell reveals sequential defects in B cell tolerance checkpoints in Sjögren's syndrome. |
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