CD3 Monoclonal Antibody (OKT3), Super Bright 600, eBioscience™
货号：63-0037-41,63-0037-42
规格：25TESTS,100TESTS
价格：1694,3492
产品类型：流式抗体
品牌：eBioscience
抗原：CD3
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：OKT3
荧光染料：Super Bright 600
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 用法： | 5uL（0.25 µg）/test(Flow） |
| Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Antibody clones OKT3 and SK7 see different epitopes.Applications Reported: This OKT3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. 00-4400) to minimize any non-specific polymer interactions.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser |
| 数据 |
CD3 Antibody (63-0037-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG2a K Isotype Control Super Bright 600 (Product # 63-4724-80) (left) or Anti-Human CD3 Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Stem cells internationalUmbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria....View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer2.Oncology lettersExpression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3.Blood cancer journalA novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer4.PloS oneTL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.5.Proceedings of the National Academy of Sciences of the United States of AmericaFunctional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination. |
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