ANTI-HUMAN CD90 (EBIO5E10) APC

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ANTI-HUMAN CD90 (EBIO5E10) APC

货号:17-0909-41,17-0909-42

规格:25T,100T

价格:1442,3090

产品类型:流式抗体

品牌:eBioscience

抗原:CD90

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:EBIO5E10

荧光染料:APC

抗体类型:单抗同型对照:IgG1, kappa
免疫原性:用法:5uL(0.25 µg)/test(Flow)
Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD90 (Thy-1) Antibody (17-0909-41) in FlowStaining of the HEL cell line with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (open histogram) or Anti-Human CD90 (Thy-1) APC (filled histogram). Total viable cells were used for analysis.
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参考文献:
1.NPJ Regenerative medicineCRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.2018"17-0909 was used in Flow cytometry/Cell sorting to showcase the therapeutic potential of gene-editing coupled with stem cell technology for the treatment of recessive dystrophic epidermolysis bullosa."Authors Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J2.Journal of translational medicineEndothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.2017"17-0909 was used in Flow cytometry/Cell sorting to demonstrate that functional endothelial and smooth muscle cells can be obtained from an available source of post-surgically discarded cardiac tissue from the right atrial appendage and right ventricular myocardium."Authors Zakharova IS,Zhiven' MK,Saaya SB,Shevchenko AI,Smirnova AM,Strunov A,Karpenko AA,Pokushalov EA,Ivanova LN,Makarevich PI,Parfyonova YV,Aboian E,Zakian SM3.Proceedings of the National Academy of Sciences of the United States of AmericaDevelopment of a diverse human T-cell repertoire despite stringent restriction of hematopoietic clonality in the thymus.2015"17-0909 was used in Flow cytometry/Cell sorting to study T-cell development in the thymus at a clonal level, with an aim to improve immune reconstitution following hematopoietic stem cell transplant."Authors Brugman MH,Wiekmeijer AS,van Eggermond M,Wolvers-Tettero I,Langerak AW,de Haas EF,Bystrykh LV,van Rood JJ,de Haan G,Fibbe WE,Staal FJ4.The Journal of clinical investigationLysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.2015"17-0909 was used in Flow cytometry/Cell sorting to demonstrate that epithelial tumourigenesis is accompanied by changes in the biochemical type of collagen cross-links."Authors Chen Y,Terajima M,Yang Y,Sun L,Ahn YH,Pankova D,Puperi DS,Watanabe T,Kim MP,Blackmon SH,Rodriguez J,Liu H,Behrens C,Wistuba II,Minelli R,Scott KL,Sanchez-Adams J,Guilak F,Pati D,Thilaganathan N,Burns AR,Creighton CJ,Martinez ED,Zal T,Grande-Allen KJ,Yamauchi M,Kurie JM5.Nucleic acids researchMulti-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.2015"17-0909 was used in Flow cytometry/Cell sorting to assess the efficiency of homologous recombination after trialling different targeting vector design parameters."Authors Byrne SM,Ortiz L,Mali P,Aach J,Church GM

技术参数

产品应用 Flow

细胞类型 MSC

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