ANTI-HUMAN CD90 (EBIO5E10) PERCP-CY5.5

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ANTI-HUMAN CD90 (EBIO5E10) PERCP-CY5.5

货号:45-0909-41,45-0909-42

规格:25T,100T

价格:1813,3603

产品类型:流式抗体

品牌:eBioscience

抗原:CD90

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:EBIO5E10

荧光染料:PerCP-Cy5.5

抗体类型:单抗同型对照:IgG1, kappa
免疫原性:用法:5uL(0.125 µg)/test(Flow)
Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometrric analysis of human erythroleukemia (HEL) cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD90 (Thy-1) Antibody (45-0909-41) in FlowStaining of the HEL cell line with Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (open histogram) or Anti-Human CD90 (Thy-1) PerCP-Cyanine5-5 (filled histogram). Total viable cells were used for analysis.
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参考文献:
1.PloS oneSpontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold.2016"45-0909 was used in Flow cytometry/Cell sorting to investigate the combination of MSCs and NFs as a therapeutic technique for the repair of joints affected by bone and cartilage destruction."Authors Sonomoto K,Yamaoka K,Kaneko H,Yamagata K,Sakata K,Zhang X,Kondo M,Zenke Y,Sabanai K,Nakayamada S,Sakai A,Tanaka Y2.PloS oneIdentification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.2012"45-0909 was used in Flow cytometry/Cell sorting to provide evidence of heterogeneity among bone marrow stromal cells and identify potential candidate proteins for quantifying MSCs in vitro."Authors Rosu-Myles M,She YM,Fair J,Muradia G,Mehic J,Menendez P,Prasad SS,Cyr TD3.The Journal of experimental medicineExpression of Thy-1 on human hematopoietic progenitor cells.1993Authors Craig W,Kay R,Cutler RL,Lansdorp PM4.BloodThy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood.1994Authors Mayani H,Lansdorp PM

技术参数

产品应用 Flow;IP;WB;MISC

细胞类型 MSC

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