ANTI-HUMAN CD45 (2D1) ALEXA FLUOR 700
货号：56-9459-41,56-9459-42
规格：25T,100T
价格：1055,2657
产品类型：流式抗体
品牌：eBioscience
抗原：CD45
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：2D1
荧光染料：Alexa Fluor 700
| 抗体类型: | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 700 |
| 免疫原性： | | 用法： | 5uL（0.25µg）/test(Flow） |
| Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD45 Antibody (56-9459-41) in FlowStaining of normal human peripheral blood cells Mouse IgG1 K Isotype Control Alexa Fluor® 700 (Product # 56-4714-80) (left) or Anti-Human CD45 Alexa Fluor® 700 (right). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Journal of immunology (Baltimore, Md. : 1950)A deletion in the gene encoding the CD45 antigen in a patient with SCID.2001Authors Tchilian EZ,Wallace DL,Wells RS,Flower DR,Morgan G,Beverley PC2.Journal of the National Cancer InstituteSubpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.1980Authors Bradstock KF,Janossy G,Pizzolo G,Hoffbrand AV,McMichael A,Pilch JR,Milstein C,Beverley P,Bollum FJ |
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