ANTI-HUMAN CD45 (2D1) FITC

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ANTI-HUMAN CD45 (2D1) FITC

货号:11-9459-42,11-9459-41

规格:100T,25T

价格:2454,1166

产品类型:流式抗体

品牌:eBioscience

抗原:CD73

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:2D1

荧光染料:FITC

抗体类型:单抗同型对照:Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC,
免疫原性:用法:5uL(1 µg)/test(Flow)
Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD45 Antibody (11-9459-42) in FlowNormal human peripheral blood cells with stained with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD45 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.
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参考文献:
1.Stem cell research and therapyVitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells.2016"11-9459 was used in Flow cytometry/Cell sorting to evaluate whether porcine adipose-derived MSCs can metabolise and respond to vitamin D."Authors Valle YL,Almalki SG,Agrawal DK2.Stem cells internationalA Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.2016"11-9459 was used in Flow cytometry/Cell sorting to determine NR2F2 plays an important role in the stemness maintenance of bone marrow-derived mesenchymal stem cells."Authors Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H3.Stem cell reportsMIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.2016"11-9459 was used in Flow cytometry/Cell sorting to show MIF plays a role in regulating the differentiation fate of human cartilage endplate stem cells under hypoxia."Authors Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y4.Tissue engineering. Part ADifferentiation of human adipose-derived stem cells into neuron-like cells which are compatible with photocurable three-dimensional scaffolds.2014"11-9459 was used in Immunocytochemistry to investigate the differentiation of human adipose-derived stem cells into neuron-like cells which are compatible with photocurable 3D scaffolds."Authors Gao S,Zhao P,Lin C,Sun Y,Wang Y,Zhou Z,Yang D,Wang X,Xu H,Zhou F,Cao L,Zhou W,Ning K,Chen X,Xu J5.PloS oneHuman and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.2014"11-9459 was used in Magnetic cell separation to investigate how the biology of mouse and human satellite cells compare."Authors Bareja A,Holt JA,Luo G,Chang C,Lin J,Hinken AC,Freudenberg JM,Kraus WE,Evans WJ,Billin AN

技术参数

产品应用 Flow;ICC;MISC

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