Mouse anti Rat CD147 antibody, clone OX-47
货号：MCA729PE
规格：100 Tests
价格：3553
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD147
物种：大鼠
宿主：小鼠
抗体亚型：IgG1
克隆号：OX-47
荧光染料：PE
| 产品介绍 | | |
| Mouse anti Rat CD147 antibody, clone OX-47recognizes a membrane glycoprotein of ~42-48 kDa expressed at high levels on activated lymphocytes but lower levels on resting cells (Patersonet al.1987). CD147 is also present on some endothelia (Fossumet al.1991). The OX-47 antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue (Fossumet al.1991).CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting (Fodaet al.2001). |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells from the NS0/1 mouse myeloma cell line. |
| Immunogen | T blasts from a mixed lymphocyte reaction |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
| 文献 |
| 1.Paterson DJet al.(1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.Mol Immunol. 24 (12): 1281-90.2.Fossum, S.et al.(1991) The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence.Eur J Immunol. 21 (3): 671-9.3.Cassella, J.P.et al.(1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels.J Anat. 189 ( Pt 2): 407-15.4.Foda, H.D.et al.(2001) Ventilator-induced lung injury upregulates and activates gelatinases and EMMPRIN: attenuation by the synthetic matrix metalloproteinase inhibitor, Prinomastat (AG3340).Am J Respir Cell Mol Biol. 25 (6): 717-24. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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