ANTI-HUMAN CD45 (HI30) APC

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ANTI-HUMAN CD45 (HI30) APC

货号:17-0459-42,17-0459-41

规格:100T,25T

价格:1197,1145

产品类型:流式抗体

品牌:eBioscience

抗原:CD45

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:HI30

荧光染料:APC

抗体类型:单抗同型对照:Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC
免疫原性:用法:5uL(0.06µg)/test(Flow)
Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD45 Antibody (17-0459-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD45 APC (purple histogram). Cells in the lymphocyte gate were used for analysis.
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参考文献:
1.Journal of translational medicineEndothelium-targeted human Delta-like 1 enhances the regeneration and homing of human cord blood stem and progenitor cells.2016"17-0459 was used in Flow cytometry/Cell sorting to investigate the effects of endothelium-targeted human Delta-like 1 on human umbilical cord blood hematopoietic stem and progenitor cells."Authors Tian DM,Liang YM,Zhang YQ2.PloS oneRapid accumulation of CD14+CD11c+ dendritic cells in gut mucosa of celiac disease after in vivo gluten challenge.2012"17-0459 was used in Flow cytometry/Cell sorting to study the varying dendritic cell subset responses within the celiac disease lesion using a model of in vivo gluten challenge."Authors Beitnes AC,Ráki M,Brottveit M,Lundin KE,Jahnsen FL,Sollid LM3.The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung DiseaseA new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.2008"17045942 was used in flow cytometry to demonstrate that aerosolized interferon-gamma results in the transient conversion of sputum smears in multidrug-resistant pulmonary tuberculosis"Authors Grahmann PR,Braun RK

技术参数

产品应用 Flow

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