ANTI-HUMAN CD45 (HI30) APC-EFLUOR 780

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ANTI-HUMAN CD45 (HI30) APC-EFLUOR 780

货号:47-0459-42,47-0459-41

规格:100T,25T

价格:3286,1288

产品类型:流式抗体

品牌:eBioscience

抗原:CD45

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:HI30

荧光染料:APC-eFluor 780

抗体类型:单抗同型对照:Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor 780
免疫原性:用法:5uL(0.125µg)/test(Flow)
Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD45 Antibody (47-0459-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (blue histogram) or Anti-Human CD45 APC-eFluor® 780 (purple histogram). Cells in the lymphocyte gate were used for analysis.
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参考文献:
1.NPJ Regenerative medicineCRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.2018"47-0459 was used in Flow cytometry/Cell sorting to showcase the therapeutic potential of gene-editing coupled with stem cell technology for the treatment of recessive dystrophic epidermolysis bullosa."Authors Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J2.Nature communicationsSF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.2015"47-0459 was used in Flow cytometry/Cell sorting to demonstrate that mutations to SF3B1 can arise from CD34(+)CD38(-)CD45RA(-)CD90(+)CD49f(+) haematopoietic stem cells and is an initiating event in myelodysplastic syndrome patients."Authors Mian SA,Rouault-Pierre K,Smith AE,Seidl T,Pizzitola I,Kizilors A,Kulasekararaj AG,Bonnet D,Mufti GJ3.DiabetesT cell-derived IL-22 amplifies IL-1β-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes.2014"47-0459 was used in Flow cytometry/Cell sorting to investigate the contribution of different cytokines to the proinflammatory signature of adipose tissue macrophages in type 2 diabetic obese patients."Authors Dalmas E,Venteclef N,Caer C,Poitou C,Cremer I,Aron-Wisnewsky J,Lacroix-Desmazes S,Bayry J,Kaveri SV,Clément K,André S,Guerre-Millo M4.NatureReprogramming human endothelial cells to haematopoietic cells requires vascular induction.2014"47-0459 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification."Authors Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

技术参数

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