ANTI-HUMAN CD45 (HI30) BIOTIN
货号:13-0459-82
规格:100 ug
价格:1873
产品类型:流式抗体
品牌:eBioscience
抗原:CD45
物种:人
宿主:小鼠
抗体亚型:IgG
克隆号:HI30
荧光染料:Biotin
抗体类型: | 单抗 | 同型对照: | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin |
免疫原性: | | 用法: | 0.25µg/test(Flow) |
Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: The HI30 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered. |
数据 |
CD45 Antibody (13-0459-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD45 PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis. |
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参考文献: |
1.Journal of neuropathology and experimental neurologyActivation status of human microglia is dependent on lesion formation stage and remyelination in multiple sclerosis.2015"13-0459 was used in Flow cytometry/Cell sorting to define the differential activation states of microglia during lesion development in multiple sclerosis-affected CNS tissues."Authors Peferoen LA,Vogel DY,Ummenthum K,Breur M,Heijnen PD,Gerritsen WH,Peferoen-Baert RM,van der Valk P,Dijkstra CD,Amor S2.Molecular therapy. Nucleic acidsRNAi-Mediated CCR5 Knockdown Provides HIV-1 Resistance to Memory T Cells in Humanized BLT Mice.2015"13-0459 was used in Flow cytometry/Cell sorting to demonstrate the potential for effective resistance of sh1005-modified CD4+ T cells to HIV-1 in humanised mice."Authors Shimizu S,Ringpis GE,Marsden MD,Cortado RV,Wilhalme HM,Elashoff D,Zack JA,Chen IS,An DS |
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