ANTI-HUMAN CD45 (HI30) PURIFIED

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ANTI-HUMAN CD45 (HI30) PURIFIED

货号:14-0459-82,14-0459-37,14-0459-95

规格:100ug,2 mg,10 mg

价格:1176,11920,29918

产品类型:流式抗体

品牌:eBioscience

抗原:CD45

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:HI30

荧光染料:其它

抗体类型:单抗同型对照:
免疫原性:用法:5uL(0.25µg)/test(Flow)
Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The HI30 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.Applications Tested: The HI30 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.
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参考文献:
1.PloS oneMonocytes from chronic HBV patients react in vitro to HBsAg and TLR by producing cytokines irrespective of stage of disease.2015"14-0459 was used in Flow cytometry/Cell sorting to investigate the in vitro and in vivo effects of hepatitis B virus surface antigen on monocytes from chronic HBV patients."Authors Boltjes A,Groothuismink ZM,van Oord GW,Janssen HL,Woltman AM,Boonstra A2.British journal of cancerPrognostic significance of CD44 variant 2 upregulation in colorectal cancer.2014"14-0459 was used in Flow cytometry/Cell sorting to investigate the clinical significance of CD133 and CD44 as putative cancer stem cell markers."Authors Ozawa M,Ichikawa Y,Zheng YW,Oshima T,Miyata H,Nakazawa K,Guan HB,Shiozawa M,Akaike M,Watanabe K,Ota M,Fujii S,Kunisaki C,Ishikawa T,Tanaka K,Akiyama H,Endo I,Taniguchi H3.American journal of respiratory and critical care medicineLung dendritic cell expression of maturation molecules increases with worsening chronic obstructive pulmonary disease.2009"14-0459 was used in Flow cytometry/Cell sorting to determine the expression of maturation molecules by individual DC subsets in relationship to COPD stage."Authors Freeman CM,Martinez FJ,Han MK,Ames TM,Chensue SW,Todt JC,Arenberg DA,Meldrum CA,Getty C,McCloskey L,Curtis JL4.Infection and immunityC-terminal repeats of Clostridium difficile toxin A induce production of chemokine and adhesion molecules in endothelial cells and promote migration of leukocytes.2008"14-0459 was used in Flow cytometry/Cell sorting to investigate how Clostridium difficile toxin A may bind to multiple carbohydrate motifs to exert its biological activity on human endothelial cells."Authors Yeh CY,Lin CN,Chang CF,Lin CH,Lien HT,Chen JY,Chia JS5.The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung DiseaseA new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.2008"14045982 was used in flow cytometry to demonstrate that aerosolized interferon-gamma results in the transient conversion of sputum smears in multidrug-resistant pulmonary tuberculosis"Authors Grahmann PR,Braun RK

技术参数

产品应用 Flow;IHC;ICC;IF

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