ANTI-HU CD105 (ENDOGLIN)(SN6) PE-CY7
货号：25-1057-41,25-1057-42
规格：25T,100T
价格：1280,3198
产品类型：流式抗体
品牌：eBioscience
抗原：CD105
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：SN6
荧光染料：PE-Cy7
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 5uL（0.125 µg）/test(Flow） |
| Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.Applications Reported: This SN6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of the U937 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD105 (Endoglin) Antibody (25-1057-41) in FlowStaining of U937 cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD105 (Endoglin) PE-Cyanine7 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis. |
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| 间充干细胞MSC分选间充干细胞MSC扩增、分化培养基CD105流式抗体细胞因子 |
| 参考文献： |
| 1.PloS oneSpontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold.2016"25-1057 was used in Flow cytometry/Cell sorting to investigate the combination of MSCs and NFs as a therapeutic technique for the repair of joints affected by bone and cartilage destruction."Authors Sonomoto K,Yamaoka K,Kaneko H,Yamagata K,Sakata K,Zhang X,Kondo M,Zenke Y,Sabanai K,Nakayamada S,Sakai A,Tanaka Y |
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