Anti-human CD34 (4H11) FITC

货号：11-0349-41,11-0349-42

规格：25TESTS,100TESTS

价格：1069,2175

产品类型：流式抗体

品牌：eBioscience

抗原：CD34

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：4H11

荧光染料：FITC

类型:	单抗	同型对照：	IgG1, kappa
浓度：	5 µL/Test	用法：	5uL（0.5 µg）/test(Flow）

产品详细信息Description: The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.Applications Reported: This 4H11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

数据

CD34 Antibody (11-0349-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD45 APC (Product # 17-0459-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD34 FITC (right). Total viable cells were used for analysis.

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参考文献：

1.Scientific reportsPotency of umbilical cord blood- and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing."11-0349 was used in Flow cytometry/Cell sorting to determine that umbilical cord blood offers a potential source of MSCs for therapeutic scarless wound healing."Authors Doi H,Kitajima Y,Luo L,Yan C,Tateishi S,Ono Y,Urata Y,Goto S,Mori R,Masuzaki H,Shimokawa I,Hirano A,Li TS2.Scientific reportsPotency of umbilical cord blood- and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing."11-0349 was used in Flow cytometry/Cell sorting to determine that umbilical cord blood offers a potential source of MSCs for therapeutic scarless wound healing."Authors Doi H,Kitajima Y,Luo L,Yan C,Tateishi S,Ono Y,Urata Y,Goto S,Mori R,Masuzaki H,Shimokawa I,Hirano A,Li TS3.Stem cell reportsMIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia."11-0349 was used in Flow cytometry/Cell sorting to show MIF plays a role in regulating the differentiation fate of human cartilage endplate stem cells under hypoxia."Authors Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y4.Scientific reportsFocal Adhesion Kinase Signaling Mediated the Enhancement of Osteogenesis of Human Mesenchymal Stem Cells Induced by Extracorporeal Shockwave."11-0349 was used in Flow cytometry/Cell sorting to investigate the underlying mechanisms of osteogenic effects of bone marrow mesenchymal stem cells induced by extracorporeal shockwave."Authors Hu J,Liao H,Ma Z,Chen H,Huang Z,Zhang Y,Yu M,Chen Y,Xu J5.EndocrinologyDifferences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome....View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer

技术参数

产品应用 Flow；MISC；FN

细胞类型 HSC

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