| Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.Applications Reported: This HIT2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.HaematologicaA distinct plasmablast and naïve B-cell phenotype in primary immune thrombocytopenia."25-0389 was used in Flow cytometry/Cell sorting to characterise the B- and T- cell compartments in patients with primary immune thrombocytopenia."Authors Flint SM,Gibson A,Lucas G,Nandigam R,Taylor L,Provan D,Newland AC,Savage CO,Henderson RB2.BiogerontologyDevelopment of depressive symptoms post hip fracture is associated with altered immunosuppressive phenotype in regulatory T and B lymphocytes."25-0389 was used in Flow cytometry/Cell sorting to investigate whether hip fracture and depressive symptoms had additive effects upon the aged immune system."Authors Duggal NA,Upton J,Phillips AC,Lord JM3.The Journal of clinical investigationEndothelium and NOTCH specify and amplify aorta-gonad-mesonephros-derived hematopoietic stem cells."25-0389 was used in Flow cytometry/Cell sorting to investigate the critical niche components and resident signals required for hematopoietic stem cell induction and self-renewal ex vivo."Authors Hadland BK,Varnum-Finney B,Poulos MG,Moon RT,Butler JM,Rafii S,Bernstein ID4.Proceedings of the National Academy of Sciences of the United States of AmericaDevelopment of a diverse human T-cell repertoire despite stringent restriction of hematopoietic clonality in the thymus."25-0389 was used in Flow cytometry/Cell sorting to study T-cell development in the thymus at a clonal level, with an aim to improve immune reconstitution following hematopoietic stem cell transplant."Authors Brugman MH,Wiekmeijer AS,van Eggermond M,Wolvers-Tettero I,Langerak AW,de Haas EF,Bystrykh LV,van Rood JJ,de Haan G,Fibbe WE,Staal FJ5.BioResearch open accessSustained Engraftment of Cryopreserved Human Bone Marrow CD34(+) Cells in Young Adult NSG Mice."25-0389 was used in Flow cytometry/Cell sorting to modify a model of the repopulating potential of hematopoietic stem cells to be able to use adult mice instead of newborns."Authors Wiekmeijer AS,Pike-Overzet K,Brugman MH,Salvatori DC,Egeler RM,Bredius RG,Fibbe WE,Staal FJ |
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