Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488/羊抗鼠IgG (H+L)交叉吸附荧光二抗，Alexa Fluor 488

货号：A-11001

规格：500µL

价格：3191

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 488

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	1µg/mL(ICC);1µg/mL(IF);1-10µg/mL(Flow)

产品详细信息To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human IgG and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins. For a highly cross-adsorbed secondary antibody equivalent, please see product Cat. No. A11029.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.靶标信息We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11001) in IFFilamentous structures of neuronal cells in a rat cerebellum were fluorescently labeled to differentiate the cell types. The cerebellum section was probed with primary antibodies to neurofilament and glial fibrillary acidic proteins (GFAP) and subsequently visualized with the green-fluorescent Alexa Fluor® 488 Goat Anti-Mouse IgG (Product # A-11001) and red-orange-fluorescent Alexa Fluor® 568 Goat Anti-Rabbit IgG (Product # A-11011) antibodies. This confocal micrograph was contributed by Gillian Davidson, Andrew Hubbard and Chris Guerin, Neurotoxicology Group, M.R.C Toxicology Unit, University of Leicester, Leicester, U.K. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11001) in IFFormation of the cephalic furrow in the anterior end of a developing Drosophila melanogaster embryo visualized with the help of several fluorescent stains. A primary antibody to neurotactin was visualized using a red-fluorescent Cy3 dye secondary antibody (Amersham Pharmacia Biotech Ltd.). Primary antibodies to plasma membrane-bound myosin and to nuclear-localized even-skipped (Eve) protein were visualized with green-fluorescent Alexa Fluor® 488 Goat Anti-Mouse IgG antibody (Product # A-11001) and Alexa Fluor® 488 Goat Anti-Rabbit IgG antibody (Product # A-11008), respectively. The nuclei were stained with blue-fluorescent Hoechst 33342 (Product # H1399, H3570, H21492). The sample was prepared by Eric Wieschaus, and the imaging was performed by Joe Goodhouse of Princeton University. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11001) in IFSimultaneous detection of expression of five genes in a whole-mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes. Red: sog labeled using aminoallyl UTP (Product # A21663, A32765) and Alexa Fluor® 647 succinimidyl ester (Product # A-20006, A20106). Green: ind labeled with DNP, followed by rabbit anti-dinitrophenyl-KLH IgG antibody (Product # A-6430) prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (Product # Z-25305). Blue: en labeled with biotin and detected with HRP-streptavidin and Alexa Fluor® 405 tyramide (TSA™ Kit 39, Product # T30952). Yellow: wg labeled with digoxigenin and detected with sheep anti-digoxigenin IgG antibody and Alexa Fluor® 594 Donkey Anti-Sheep IgG antibody (Product # A-11016). Magenta: msh labeled with fluorescein and detected with mouse anti-fluorescein/Oregon Green® IgG2a antibody (Product # A-6421) and Alexa Fluor® 488 Goat Anti-Mouse IgG antibody (Product # A-11001, A11029). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Frontiers in microbiologyHELZ2 Is an IFN Effector Mediating Suppression of Dengue Virus.2.International journal of biological sciencesRegulatory Axis of miR-195/497 and HMGA1-Id3 Governs Muscle Cell Proliferation and Differentiation.3.PloS onePEG3 Interacts with KAP1 through KRAB-A.4.Nature protocolsGeneration of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos.5.Scientific reportsOverexpression of human NR2B receptor subunit in LMAN causes stuttering and song sequence changes in adult zebra finches.

技术参数

产品优点 1.特异性强； 2.亮度高，耐淬灭，对PH值不敏感。

产品应用 Flow；ICC；IF；IHC；MISC；IHC (Free)；IHC (P)；WB；IHC (F)

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-16762.htm