HA Tag Monoclonal Antibody (2-2.2.14) HA标签抗体

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HA Tag Monoclonal Antibody (2-2.2.14) HA标签抗体

货号:26183

规格:100 µg

价格:2546

产品类型:标签抗体

品牌:Thermo Fisher

物种:Tag

宿主:小鼠

抗体亚型:IgG

克隆号:2-2.2.14

荧光染料:其它

点击Invitrogen标签抗体集,查看更多标签抗体

抗体类型:单抗同型对照: lgG1
免疫原性:HA 肽段YPYDVPDVPDYA derivitized to ovalbulmin用法:

IP;

0.1 µg/mL(WB)

Product Specific InformationHA标签抗体26183特异性识别HA 肽段YPYDVPDYA。此抗体已成功应用于WB和免疫共沉淀IP实验。HA 合成多肽 ( # 26184)可用作对照或者用于中和实验中,同时可在亲和层析中用于竞争性洗脱HA标签融合蛋白。HA Tag (血凝素标签)是一种表位标签,其提供了一种在多种类型细胞中定位基因表达的方法,用于研究蛋白-蛋白相互作用的拓扑学结构、鉴定无特异性抗体的相关蛋白、鉴定新确认的低丰度和低免疫原性蛋白等。HA(血凝素)是源自流感血凝素的表面糖蛋白,并且通常与蛋白质一起表达为标签,以帮助鉴定和纯化用于功能分析的标签融合蛋白质。 HA标签抗体可用于标签蛋白的蛋白质检测或免疫共沉淀。
数据

HA Tag Antibody (26183) in WBWestern blot analysis of HA Epitope Tag was performed by various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HA Epitope Tag monoclonal antibody (Product # 26183) at a dilution of 1:10,000 for 1 hour at room temperature on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
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参考文献:
1.Proceedings of the National Academy of Sciences of the United States of America-Rock2 pathway controls dendritic integrity and memory.2017. Authors Bobo-Jiménez V,Delgado-Esteban M,Angibaud J,Sánchez-Morán I,de la Fuente A,Yajeya J,Nägerl UV,Castillo J,Bolaños JP,Almeida A2.PloS oneThe 9aaTAD Is Exclusive Activation Domain in Gal4.2017.Authors Piskacek M,Havelka M,Rezacova M,Knight A3.NatureeIF3d is an mRNA cap-binding protein that is required for specialized translation initiation.2016.Authors Lee AS,Kranzusch PJ,Doudna JA,Cate JH4.The EMBO journalLoss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.2016.Authors Sellier C,Campanari ML,Julie Corbier C,Gaucherot A,Kolb-Cheynel I,Oulad-Abdelghani M,Ruffenach F,Page A,Ciura S,Kabashi E,Charlet-Berguerand N5.Nature communicationsStructural insights of ZIP4 extracellular domain critical for optimal zinc transport.2016.Authors Zhang T,Sui D,Hu J

技术参数

产品应用 WB;ChIP;IP;MISC;ICC

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