Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488/羊抗鼠 IgG (H+L) 高交叉吸附荧光二抗，AF488

货号：A-11029

规格：500µL

价格：3682

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 488

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	1-10µg/mL(ICC);1-10µg/mL（IF);1-10µg/mL(IHC)

产品详细信息To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been highly cross-adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in 'highly cross-adsorbed' preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.靶标信息We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11029) in IFIntermediate filaments of astrocytes and ependymal cells in a mouse brain cryosection identified using mouse monoclonal anti-GFAP and visualized with Alexa Fluor® 488 goat anti-mouse IgG antibody. Intermediate filaments of astrocytes and ependymal cells in a 14 µm mouse brain cryosection were identified using mouse monoclonal anti-glial fibrillary monoclonal antibody (anti-GFAP, Product # A-21282) and visualized with green-fluorescent Alexa Fluor® 488 goat anti-mouse IgG antibody (Product # A-11029). Nuclei were stained with blue-fluorescent DAPI (Product # D1306, D3571, D21490). The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/). 3-D reconstruction was performed using Imaris software (Bitplane AG, http://www.bitplane.com/). Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11029) in IFSimultaneous detection of expression of five genes in a whole-mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes. Red: sog labeled using aminoallyl UTP (Product # A21663, A32765) and Alexa Fluor® 647 succinimidyl ester (Product # A-20006, A20106). Green: ind labeled with DNP, followed by rabbit anti-dinitrophenyl-KLH IgG antibody (Product # A-6430) prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (Product # Z-25305). Blue: en labeled with biotin and detected with HRP-streptavidin and Alexa Fluor® 405 tyramide (TSA™ Kit 39, Product # T30952). Yellow: wg labeled with digoxigenin and detected with sheep anti-digoxigenin IgG antibody and Alexa Fluor® 594 Donkey Anti-Sheep IgG antibody (Product # A-11016). Magenta: msh labeled with fluorescein and detected with mouse anti-fluorescein/Oregon Green® IgG2a antibody (Product # A-6421) and Alexa Fluor® 488 Goat Anti-Mouse IgG antibody (Product # A-11001, A11029). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego. Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11029) in IFHDFn cells were fixed, permeabilized, and blocked using the Image-iT® Fixation/Permeabilization Kit (Product # R37602). Golgi were labeled with an anti-golgin 97 antibody (Product # A-21270) followed by detection with an Alexa Fluor® 488 secondary (Product # A-11029). Cells were also stained with Alexa Fluor® 594 phalloidin (Product # A12381) to label actin and NucBlue™ Fixed (Product # R37606) to label nuclei. Finally, cells were mounted in ProLong® Gold antifade reagent.

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参考文献：

1.AutophagyUbiquitin ligase SYVN1/HRD1 facilitates degradation of the SERPINA1 Z variant/α-1-antitrypsin Z variant via SQSTM1/p62-dependent selective autophagy.2.Breast cancer research : BCRLoss of MMP-8 in ductal carcinoma in situ (DCIS)-associated myoepithelial cells contributes to tumour promotion through altered adhesive and proteolytic function.3.Scientific reportsBacterial secretion system skews the fate of Legionella-containing vacuoles towards LC3-associated phagocytosis.4.Scientific reportsIdentification of Elongated Primary Cilia with Impaired Mechanotransduction in Idiopathic Scoliosis Patients.5.Journal of translational medicineEndothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.

技术参数

产品应用 Flow；ICC；IF；IHC；Flow；MISC；IHC (Free)；IHC (P)；WB；IHC (F)

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