CD3e Monoclonal Antibody (145-2C11), PE-eFluor 610
货号：61-0031-82,61-0031-80
规格：100μg,25μg
价格：2895,1164
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：Armenian hamster仓鼠
抗体亚型：IgG
荧光染料：PE-eFluor 610
| 抗体类型 | 单抗 | 同型对照： | Armenian Hamster IgG Isotype Control (eBio299Arm), PE-eFluor 610 |
| 浓度： | 0.2 mg/mL | 用法： | 1 µg/test (Flow) |
| 产品详细信息Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency |
| 数据 |
| CD3e Antibody (61-0031-82) in FlowStaining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.5 µg of Armenian Hamster IgG Isotype Control PE-eFluor® 610 (Product # 61-4888-82) (left) or 0.5 µg of Anti-Mouse CD3e PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.International journal of cancerHSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma.AuthorsGeng H,Zhang GM,Xiao H,Yuan Y,Li D,Zhang H,Qiu H,He YF,Feng ZH2.Investigative ophthalmology and visual scienceCCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response.AuthorsTakeuchi A,Usui Y,Takeuchi M,Hattori T,Kezuka T,Suzuki J,Okunuki Y,Iwasaki T,Haino M,Matsushima K,Usui M3.Frontiers in immunologyTpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells."Published figure using CD3e monoclonal antibody (Product # 61-0031-82) in Flow Cytometry"4.Journal of cellular and molecular medicineEnhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells."Published figure using CD3e monoclonal antibody (Product # 61-0031-82) in Flow Cytometry"5.The Journal of neuroscience : the official journal of the Society for NeuroscienceSpinal Motor Circuit Synaptic Plasticity after Peripheral Nerve Injury Depends on Microglia Activation and a CCR2 Mechanism."61-0031 was used in Flow cytometry/Cell sorting to show that both activation of ventral microglia and a CCR2-dependent mechanism are necessary for removal of VGLUT1 synapses and alterations in Ia-circuit function following nerve injuries."AuthorsRotterman TM,Akhter ET,Lane AR,MacPherson KP,García VV,Tansey MG,Alvarez FJ |
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