Goat anti-Human IgG Fc Secondary Antibody, PE, eBioscience™/羊抗人IgG Fc二抗,PE,eBioscience™

2024-10-17

Goat anti-Human IgG Fc Secondary Antibody, PE, eBioscience™/羊抗人IgG Fc二抗,PE,eBioscience™

货号:12-4998-82

规格:100 µg

价格:1038

产品类型:荧光二抗

品牌:Thermo Fisher

抗原:IgG (H+L)

物种:人

宿主:山羊

抗体亚型:IgG

荧光染料:PE

点击查看所有Alexa Fluor 荧光二抗
抗体类型:荧光二抗同型对照:
浓度: 0.5 mg/mL用法:流式细胞分析 (Flow) 1 µg/test
产品信息:Description: This goat antibody reacts with the Fc fragment of heavy chains on human IgG but notwith the light chains on most human immunoglobulins. No antibody was detected against human IgM or IgA, or against nonimmunoglobulinserum proteins. This antibody has been tested to ensure minimalcross-reaction with bovine, horse, and mouse serum proteins, but the antibody may cross-react with immunoglobulins fromother species.Applications Reported: PE goat anti-Human IgG (Fc gamma Fragment Specific) has been reported for use in flow cytometric analysis.Applications Tested: PE goat anti-Human IgG (Fc gamma Fragment Specific) has been tested by flow cytometric analysis to detect purified human Fc gamma-tagged recombinant proteins. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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参考文献:
1.OncoimmunologyAntigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.2.mAbsTranspo-mAb display: Transposition-mediated B cell display and functional screening of full-length IgG antibody libraries.4.NatureNon-equivalence of Wnt and R-spondin ligands during Lgr5+ intestinal stem-cell self-renewal.5.mAbsMining the human autoantibody repertoire: isolation of potent IL17A-neutralizing monoclonal antibodies from a patient with thymoma.

技术参数

产品应用 Flow;Misc

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