CD8a Monoclonal Antibody (53-6.7), APC

货号：17-0081-81,17-0081-82,17-0081-83

规格：50μg,100μg,200μg

价格：1620,2629,3949

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：53-6.7

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: The 53-6.7 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocyte or splenocyte suspensions. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD8a Antibody (17-0081-82) in Flow Staining of mouse splenocytes with Anti-Mouse CD3 PerCP-eFluor® 710 (Product # 46-0032-82) and 0.06 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.06 µg of Anti-Mouse CD8a APC (right). Cells in the lymphocyte gate were used for analysis

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参考文献：

1. Frontiers in physiologyMacrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. mSphereMicrobiota Reconstitution Does Not Cause Bone Loss in Germ-Free Mice.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. International journal of medical sciencesT Cells Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. The Journal of experimental medicineEgr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Acta pharmacologica SinicaBetulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow

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