Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP/山羊抗小鼠IgG(H+L)二抗，HRP
货号：31430
规格：2ml
价格：1644
产品类型：二抗
品牌：Thermo Fisher
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：HRP
| 抗体类型: | 多抗 | 同型对照： | lgG |
| 浓度： | 0.8mg/mL | 用法： | 1:10,000-1:25,000(ELISA)； 1:5,000-1:100,000(IHC)； 1:500-1:5,000(IP)； 1:5,000-1:200,000(WB) |
| 产品详细信息Product # 31430 has been successfully used in Western blot, IHC and IP applications.Product # 31430 reacts with the heavy chains of mouse igg and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Reconstitute with 2.0 ml of distilled water (0.8 mg/ml after restoration).靶标信息Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
| 数据 |
Mouse IgG (H+L) Secondary Antibody (31430) in IHC (P)Immunohistochemistry was performed on deparaffinized canine fetal brain tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked with Avidin/Biotin Blocking kit (Product # 00-4303) at room temperature and then probed with a Sox2 monoclonal antibody (Product # MA1-014) at a dilution of 1:200 for one hour at room temperature. Tissues were washed extensively with TBS + 0.025% Triton X-100 (Product # 28314). Detection was performed using a goat anti-mouse HRP secondary antibody (Product # 31430) at a dilution of 1:500 followed by colorimetric detection using metal enhanced DAB (Product # 34065). Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken on a Zeiss Axiovision microscope at 20X magnification. The bottom layer with a dense staining of Sox2 is in the subependymal plate, where there are large numbers of progenitor cells. The upper layer is neuroparenchyma where cells are differentiated, therefore less staining of Sox2. Note: Data courtesy of Innovators Program. |
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| 参考文献： |
| 1.PloS oneAcute death of astrocytes in blast-exposed rat organotypic hippocampal slice cultures.2.Journal of molecular microbiology and biotechnologyMining of Ruminant Microbial Phytase (RPHY1) from Metagenomic Data of Mehsani Buffalo Breed: Identification, Gene Cloning, and Characterization.4.Journal of physiology and biochemistryComparative efficacy of alpha-linolenic acid and gamma-linolenic acid to attenuate valproic acid-induced autism-like features.5.Journal of neuroinflammationThe Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. |
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