Magic Red™ Cathepsin K Kit
货号：ICT939
规格：25 Tests
价格：2763
产品类型：细胞增殖活性检测
品牌：Bio-Rad
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| 产品介绍 | | |
| The Magic Red Cathepsin K Kit uses a quick and easy method to analyze intracellular cathepsin K protease activity in whole living cells. |
| 产品详情 | | |
| Reagents in the Kit | Magic Red Substrate (MR-LR2), 25 Tests - lyophilizedHoechst 33342, 1 mLAcridine Orange, 0.5 mL |
| Test Principle | Cathepsin Magic Red Kits measure cathepsin K protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.The Magic Red reagent contains a cathepsin K target sequence peptide (LR)2linked to a red (Cresyl Violet) fluorescent probe.The Cathepsin Magic Red Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates. |
| Approx. Protein Concentrations | Magic Red™ is a trademark of Immunochemistry Technologies, LLC. |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Store the unopened kit and each unopened component at +4℃ until the expiration date.Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20℃ for 6 months protected from light and thawed no more than twice during that time. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Immunofluorescence | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Azuma, K.et al.(2007) Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales.Biochem Biophys Res Commun. 362 (3): 594-600.2.de Vrieze, E.et al.(2010) ALP, TRAcP and cathepsin K in elasmoid scales: a role in mineral metabolism?Journal of Applied Ichthyology. 26 (2): 210-3. |
技术参数 产品应用 if
产品类型 细胞活性检测
推荐用于 if
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-33913.htm
QQ:1749072012