Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP

admin 2024-10-17 20

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP

货号:31460

规格:2ml

价格:1644

产品类型:二抗

品牌:Thermo Fisher

物种:兔

宿主:山羊

抗体亚型:IgG

荧光染料:HRP

抗体类型:多抗同型对照: lgG
免疫原性:用法: 1:5000-1:10,000(ELISA); 1:5,000-1:100,000(IHC); 1:500-1:5,000(IP); 1:10,000-1:200,000(WB)

Product Specific InformationProduct # 31460 has been successfully used in Western blot, IHC and IP applications.Product # 31460 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species and with SuperBlock® Blocking Buffers.Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Reconstitute with 2.0 ml of distilled water (0.8 mg/ml after restoration).Background/Target InformationThermo Scientific Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据

Rabbit IgG (H+L) Secondary Antibody (31460) in IHC (P)Immunohistochemistry was performed on human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave. Following antigen retrieval, tissues were blocked in 3% BSA-PBS for 30 minutes and then probed with (right panel) or without (left panel) a MuSK polyclonal antibody (Product # PA1-1741) at a dilution of 1:20 overnight at 4C in a humidified chamber. Tissues were washed extensively with TBS + 0.025% Triton X-100 (Product # 28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product # 35000) for 30 minutes at room temperature. Detection was performed using a goat anti-rabbit HRP secondary antibody (Product # 31460) followed by colorimetric detection using metal enhanced DAB (Product # 34065). Tissues were counterstained with hematoxylin and prepped for mounting.
推荐产品:
二抗荧光二抗免疫组化一抗标签抗体
参考文献:
1.Molecular neurobiologyProlyl Oligopeptidase Regulates Dopamine Transporter Phosphorylation in the Nigrostriatal Pathway of Mouse.2018.Authors Julku UH,Panhelainen AE,Tiilikainen SE,Svarcbahs R,Tammimäki AE,Piepponen TP,Savolainen MH,Myöhänen TT2.Cell chemical biologyA Ratiometric Sensor for Imaging Insulin Secretion in Single β Cells.2017.Authors Schifferer M,Yushchenko DA,Stein F,Bolbat A,Schultz C3.Antiviral researchActivity of andrographolide against dengue virus.2017.Authors Panraksa P,Ramphan S,Khongwichit S,Smith DR4.Free radical biology and medicineSixty years old is the breakpoint of human frontal cortex aging.2017.Authors Cabré R,Naudí A,Dominguez-Gonzalez M,Ayala V,Jové M,Mota-Martorell N,Piñol-Ripoll G,Gil-Villar MP,Rué M,Portero-Otín M,Ferrer I,Pamplona R5.Molecular therapy. Nucleic acidsA piRNA-like Small RNA Induces Chemoresistance to Cisplatin-Based Therapy by Inhibiting Apoptosis in Lung Squamous Cell Carcinoma.2017.Authors Wang Y,Gable T,Ma MZ,Clark D,Zhao J,Zhang Y,Liu W,Mao L,Mei Y

技术参数

产品应用 WB;ELSA;IP;IHC

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://biorod.cn/thread-16780.htm
产品询价需求提交
最新回复 (0)
返回
发布信息
扫码添加客服微信,咨询产品报价