CD44 Monoclonal Antibody (IM7), eBioscience™

货号：14-0441-81,14-0441-82,14-0441-85,14-0441-86

规格：50 µg,100 µg,500 µg,1 mg

价格：721,1304,2494,4153

产品类型：流式抗体

品牌：eBioscience

抗原：CD44

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：IM7

荧光染料：其它

抗体类型:	单抗	同型对照：
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: The IM7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistochemical staining and immunoblotting (non-reducing conditions). It has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified IM7, cat. 16-0441, in functional assays.).Applications Tested: The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD44 Antibody (14-0441-81) in FlowStaining of C57Bl/6 splenocytes with 0.06 µg of Rat IgG2b Isotype Control Purified(Product # 14-4031-82) (open histogram) or 0.06 µg of Anti-Human/Mouse CD44 Purified(filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cellswere used for analysis.

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参考文献：

1.PloS oneT-Bet and Eomes Regulate the Balance between the Effector/Central Memory T Cells versus Memory Stem Like T Cells."14-0441 was used in Flow cytometry/Cell sorting to investigate the role of T-bet and Eomes in shaping the memory T cell pool."Authors Li G,Yang Q,Zhu Y,Wang HR,Chen X,Zhang X,Lu B2.Journal of immunology (Baltimore, Md. : 1950)Monocytes."14-0441 was used in Flow cytometry/Cell sorting to investigate the mechanism of action underlying the tolerogenic function of soluble CD83."Authors Horvatinovich JM,Grogan EW,Norris M,Steinkasserer A,Lemos H,Mellor AL,Tcherepanova IY,Nicolette CA,DeBenedette MA3.PloS oneCRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development."14-0441 was used in Flow cytometry/Cell sorting to elucidate the contribution of the SLAM receptors in immunodysregulation observed in X-linked lymphoproliferative syndrome Type 1."Authors Huang B,Gomez-Rodriguez J,Preite S,Garrett LJ,Harper UL,Schwartzberg PL4.The Journal of biological chemistryThe high and low molecular weight forms of hyaluronan have distinct effects on CD44 clustering.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5.Clinical cancer research : an official journal of the American Association for Cancer ResearchEpstein-Barr virus infection and expression of B-cell oncogenic markers in HIV-related diffuse large B-cell Lymphoma."14-0441 was used in Immunohistochemistry to suggest that EBV infection was associated with expression of several tumor markers that are involved in the NF-κB pathway."Authors Chao C,Silverberg MJ,Martínez-Maza O,Chi M,Abrams DI,Haque R,Zha HD,McGuire M,Xu L,Said J

技术参数

产品应用 Flow；FN; ICC；IF；IHC；MISC；IHC (P)；WB；IHC (F); ELISA

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