CD44 Monoclonal Antibody (IM7), PE-Cyanine5, eBioscience™

货号：15-0441-81,15-0441-82,15-0441-83

规格：50 µg,100 µg,200 µg

价格：1492,1991,3650

产品类型：流式抗体

品牌：eBioscience

抗原：CD44

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：IM7

荧光染料：PE-Cy5

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine5
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: The IM7 antibody has been reported for use in flow cytometric analysis.Applications Tested: The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD44 Antibody (15-0441-81) in FlowStaining of BALB/c splenocytes with staining buffer (autofluorescence)(open histogram) or 0.06 µg of Anti-Human/Mouse CD44 PE-Cyanine5(filled histogram).Total cells were used for analysis.

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参考文献：

1.Nature communicationsA mouse model for a partially inactive obesity-associated human MC3R variant."15-0441 was used in Flow cytometry/Cell sorting to confirm the importance of MC3R signalling in human metabolism, and suggest a novel role for MC3R in adipose tissue development."Authors Lee B,Koo J,Yun Jun J,Gavrilova O,Lee Y,Seo AY,Taylor-Douglas DC,Adler-Wailes DC,Chen F,Gardner R,Koutzoumis D,Sherafat Kazemzadeh R,Roberson RB,Yanovski JA2.Molecular medicine reportsBreast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics."15-0441 was used in Flow cytometry/Cell sorting to demonstrate that breast cancer stem cells are heterogeneous, and they exhibit distinct biological characteristics."Authors Shao J,Fan W,Ma B,Wu Y3.Nature communicationsT-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer4.The Journal of clinical investigationExcessive expression of miR-27 impairs Treg-mediated immunological tolerance.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5.Journal of immunology (Baltimore, Md. : 1950)The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer

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