SSEA3 Monoclonal Antibody (MC-631)

货号：MA1-020,MA1-020X

规格：100 ug,20 uL

价格：4070,2148

产品类型：流式抗体

品牌：Thermo Fisher

抗原：SSEA3

物种：人

宿主：大鼠

抗体亚型：IgM

克隆号：MC-631

荧光染料：其它

抗体类型:	单抗	同型对照：	IgM
免疫原性：	Fischer rat immunized with 4-8 cell stage mouse embryos	用法：	1:100(Flow）;1:100-1:500(ICC);1:100-1:500(IF)

Target InformationSSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SSEA3 Antibody (MA1-020) in IFImmunofluorescent analysis of SSEA-3 using anti-SSEA-3 monoclonal antibody (Product # MA1-020) shows expression in human teratocarcinoma NCCIT cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rat monoclonal antibody recognizing SSEA-3 (Product # MA1-020), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with a goat-anti-rat IgM secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. SSEA3 Antibody (MA1-020) in FlowFlow cytometry analysis of SSEA-3 using anti-SSEA-3 monoclonal antibody (Product # MA1-020) shows positive staining of H9 human embryonic stem cells (blue line). H9 human embryonic stem cells were harvested, fixed and washed with PBS. Cells were incubated with anti-SSEA-3 monoclonal antibody (Product # MA1-020) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with fluorescein conjugated secondary antibody. 100,000 cells were stained for each sample. SSEA3 Antibody (MA1-020) in FlowFlow cytometry analysis of SSEA-3 using anti-SSEA-3 monoclonal antibody (Product # MA1-020) shows positive staining of HEL 11.4 iPS cells (blue line). HEL 11.4 iPS cells were harvested, fixed and washed with PBS. Cells were incubated with anti-SSEA-3 monoclonal antibody (Product # MA1-020) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with fluorescein conjugated secondary antibody. 100,000 cells were stained for each sample. SSEA3 Antibody (MA1-020) in IFImmunofluorescent analysis of SSEA-3 using anti-SSEA-3 monoclonal antibody (Product # MA1-020) shows staining on the cell surface of human HEL 11.4 iPS cells, indicating pluripotency. SSEA-3 staining (green) and an overlay image of SSEA-3 with DAPI (blue) is shown. HEL 11.4 iPS cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a rat monoclonal antibody recognizing SSEA-3 (Product # MA1-020) at a dilution of 1:20 overnight at 4 °C, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

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参考文献：

1.Proceedings of the National Academy of Sciences of the United States of AmericaStage-specific embryonic antigen-3 (SSEA-3) and β3GalT5 are cancer specific and significant markers for breast cancer stem cells."MA1020 was used in flow cytometry to find that breast cancer stem cells isolated with CD44(+)CD24(-/lo)SSEA-3(+) or ESA(hi)PROCR(hi)SSEA-3(+) markers have higher tumorigenicity than those with conventional markers"Authors Cheung SK,Chuang PK,Huang HW,Hwang-Verslues WW,Cho CH,Yang WB,Shen CN,Hsiao M,Hsu TL,Chang CF,Wong CH

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