CD140a (PDGFRA) Monoclonal Antibody (APA5), Biotin, eBioscience™

货号：13-1401-80,13-1401-82

规格：25 µg,100 µg

价格：838,1786

产品类型：流式抗体

品牌：eBioscience

抗原：CD140a (PDGFRA)

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：APA5

荧光染料：Biotin

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	1 µg/test(Flow）

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.Applications Reported: The APA5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationPDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD140a (PDGFRA) Antibody (13-1401-80) in FlowStaining of NIH/3T3 cells with 0.5 µg of Anti-Mouse CD140a (PDGF Receptor a) Biotin followed by Streptavidin PE (Product # 12-4317-87). Appropriate isotype controlswere used (open histogram). Total viable cells were used for analysis.

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参考文献：

1.The Journal of clinical investigationPreexisting endothelial cells mediate cardiac neovascularization after injury.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer2.eLifeClec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton."13-1401 was used in Flow cytometry/Cell sorting to study how Clec11a/Osteolectin maintains the adult skeleton by promoting the differentiation of mesenchymal progenitors into mature osteoblasts."Authors Yue R,Shen B,Morrison SJ3.PloS oneExogenous HGF Bypasses the Effects of ErbB Inhibition on Tumor Cell Viability in Medulloblastoma Cell Lines."13-1401 was used in Flow cytometry/Cell sorting to investigate the role of micro-environmental growth factors in relation to the effects of receptor tyrosine kinase inhibitors."Authors Zomerman WW,Plasschaert SL,Diks SH,Lourens HJ,Meeuwsen-de Boer T,Hoving EW,den Dunnen WF,de Bont ES4.Molecular cancer research : MCRDynamic and nuclear expression of PDGFRα and IGF-1R in alveolar Rhabdomyosarcoma.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5.Human molecular geneticsActivation of non-myogenic mesenchymal stem cells during the disease progression in dystrophic dystrophin/utrophin knockout mice.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer

技术参数

产品应用 Flow；FN；IF；ICC；IF；IHC；IHC (P)；WB；IHC (F)

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