CD45 Monoclonal Antibody (30-F11), Functional Grade, eBioscience™
货号：16-0451-85,16-0451-38,16-0451-025,16-0451-050
规格：500μg,5 mg,25 mg,50 mg
价格：2872,24089,72266,120443
产品类型：抗体和ELISA
品牌：Thermo Fisher
抗体类型: | 单抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade |
免疫原性： | | 用法： | 0.5 µg/test(Flow);Assay-Dependent(FN); Assay-Dependent(IHC);Assay-Dependent(IP) |
Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays.).Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Background/Target InformationCD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| CD45 Antibody (16-0451-85) in Flow Staining of C57Bl/6 splenocytes with 0.25 µg of Purified Rat IgG2b Isotype Control (Product # 14-4031-82) (open histogram) or 0.25 µg of Anti-Mouse CD45 Purified(filled histogram)followed by Anti-Rat IgG FITC (Product # 11-4811-85). Cells in the lymphocyte gate were used for analysis. |
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参考文献： |
| 1. Scientific reportsHeavy Chain-Hyaluronan/Pentraxin 3 from Amniotic Membrane Suppresses Inflammation and Scarring in Murine Lacrimal Gland and Conjunctiva of Chronic Graft-versus-Host Disease....2017.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer2. Journal of ophthalmologyReduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease.2017.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3. CellThe Hippo Pathway Kinases LATS1/2 Suppress Cancer Immunity.2016.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer4. Kidney internationalEndothelin A receptor activation on mesangial cells initiates Alport glomerular disease.2015.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5. Nature communicationsEndothelial Gata5 transcription factor regulates blood pressure.2015.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer |
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