CD140a (PDGFRA) Monoclonal Antibody (APA5), PE-Cyanine7, eBioscience™

货号：25-1401-80,25-1401-82

规格：25 µg,100 µg

价格：1311,3157

产品类型：流式抗体

品牌：eBioscience

抗原：CD140a (PDGFRA)

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：APA5

荧光染料：PE-Cy7

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationPDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD140a (PDGFRA) Antibody (25-1401-80) in FlowStaining of NIH/3T3 cells with 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7(Product # 25-4321-82) (blue histogram) or 0.06 µg of Anti-Mouse CD140a (PDGF Receptor a)PE-Cyanine7 (purple histogram). Total viable cells were used for analysis.

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参考文献：

1.JCI insightmTOR inhibition and BMP signaling act synergistically to reduce muscle fibrosis and improve myofiber regeneration.2.Nature communicationsGenetic lineage tracing defines myofibroblast origin and function in the injured heart.3.NatureDrug-based modulation of endogenous stem cells promotes functional remyelination in vivo.4.Proceedings of the National Academy of Sciences of the United States of AmericaOlig2+ neuroepithelial motoneuron progenitors are not multipotent stem cells in vivo.Authors Mukouyama YS,Deneen B,Lukaszewicz A,Novitch BG,Wichterle H,Jessell TM,Anderson DJ5.The Journal of neuroscience : the official journal of the Society for NeuroscienceTlx, an orphan nuclear receptor, regulates cell numbers and astrocyte development in the developing retina.Authors Miyawaki T,Uemura A,Dezawa M,Yu RT,Ide C,Nishikawa S,Honda Y,Tanabe Y,Tanabe T

技术参数

产品应用 Flow；IHC (F)

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