Goat anti-Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568/山羊抗小鼠IgM (重链)交叉吸附二抗，Alexa Fluor 568

货号：A-21043

规格：500 µg

价格：3196

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse Mu immunoglobulin

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料： Alexa Fluor 568

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抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	2 mg/mL (ICC)； 2 mg/mL (IF);1-10 µg/mL (IHC)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody (A-21043) in IF Immunofluorescence analysis of Goat anti-Mouse IgM Heavy Chain Secondary Antibody, Alexa Fluor 568 conjugate was performed using MCF-7 cells stained withHSP70 (MB-H1) Mouse Monoclonal Primary Antibody (Product # 33-3800). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Mouse primary antibody for 3 hours at room temperature. Goat anti-Mouse IgM Heavy Chain Secondary Antibody, Alexa Fluor 568 conjugate (Product # A-21043) was used at a concentration of 0.2µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of HSP70 in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody (A-21043) in ICC Immunofluorescence analysis of HSP70 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP70 (MB-H1) Mouse Monoclonal Antibody (Product # 33-3800) at 2 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 (Product # A-21043) at 0.2 microgram/mL for 45 minutes at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: green) was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300). Panel d represents the merged image showing predominant cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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参考文献：

1. Frontiers in immunologyActivation of GABAA Receptors in Colon Epithelium Exacerbates Acute Colitis.2. Experimental neurologyPseudophosphorylation of tau at S422 enhances SDS-stable dimer formation and impairs both anterograde and retrograde fast axonal transport.3. Human molecular geneticsDistinct pathways leading to TDP-43-induced cellular dysfunctions.4. Frontiers in immunologyActivation of GABAA Receptors in Colon Epithelium Exacerbates Acute Colitis.5. Cell journalMetabolic Signature of Pluripotent Stem Cells.

技术参数

产品应用 ICC;IF;IHC;Misc;IHC(Free)

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