Mouse IgG1 kappa Isotype Control, PerCP-eFluor 710

货号：46-4714-82,46-4714-80

规格：100μg,25μg

价格：2528,1038

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：PerCP-eFluor™ 710

抗体类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	Assay-Dependent (Ctrl)； Assay-Dependent (Flow)

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control. Applications Reported: The Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: The Mouse IgG1 K Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.PloS onePolycyclic aromatic hydrocarbons reciprocally regulate IL-22 and IL-17 cytokines in peripheral blood mononuclear cells from both healthy and asthmatic subjects.2016.Authors Plé C,Fan Y,Ait Yahia S,Vorng H,Everaere L,Chenivesse C,Balsamelli J,Azzaoui I,de Nadai P,Wallaert B,Lazennec G,Tsicopoulos A2. PLoS pathogensTIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.2016.Authors Chew GM,Fujita T,Webb GM,Burwitz BJ,Wu HL,Reed JS,Hammond KB,Clayton KL,Ishii N,Abdel-Mohsen M,Liegler T,Mitchell BI,Hecht FM,Ostrowski M,Shikuma CM,Hansen SG,Maurer M,Korman AJ,Deeks SG,Sacha JB,Ndhlovu LC3. Stem cells and developmentEnrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19.2015.Authors Bauderlique-Le Roy H,Vennin C,Brocqueville G,Spruyt N,Adriaenssens E,Bourette RP

技术参数

产品应用 Ctrl；Flow

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