Rat IgG2b kappa Isotype Control, eFluor 450

货号：48-4031-82,48-4031-80

规格：100 µg,25 µg

价格：1964,872

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：eB149/10H5

荧光染料：eFluor 450

抗体类型:	单抗	同型对照：	IgG2b, kappa
免疫原性：		用法：

Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: Rat IgG2b Isotype Control eFluor® 450 has been reported for use in flow cytometric analysis.Applications Tested: eFluor® 450 Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.PloS oneMedia from macrophages co-incubated with Enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology."48-4031 was used in Flow cytometry/Cell sorting to demonstrate that co-incubation of murine macrophages with E. faecalis strains leads to media capable of inducing epithelial cell monolayer reassembly."Authors Belogortseva N,Krezalek M,Guyton K,Labno C,Poroyko V,Zaborina O,Alverdy JC2.Investigative ophthalmology and visual scienceCD11b+GR1+ myeloid cells secrete NGF and promote trigeminal ganglion neurite growth: implications for corneal nerve regeneration."48-4031 was used in Flow cytometry/Cell sorting to study trigeminal ganglion neurites using mice with fluorescent bone marrow cells to show increased growth when CD11b+GR1+ myeloid cells secrete NGF."Authors Sarkar J,Chaudhary S,Jassim SH,Ozturk O,Chamon W,Ganesh B,Tibrewal S,Gandhi S,Byun YS,Hallak J,Mahmud DL,Mahmud N,Rondelli D,Jain S3.Arteriosclerosis, thrombosis, and vascular biologyMonocyte chemoattractant protein 1-mediated migration of mesenchymal stem cells is a source of intimal hyperplasia."48-4031 was used as a Control in experiments to identify the predominant cell types associated with transplant arteriosclerosis and the factors inducing their recruitment."Authors Grudzinska MK,Kurzejamska E,Bojakowski K,Soin J,Lehmann MH,Reinecke H,Murry CE,Soderberg-Naucler C,Religa P

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