CD11b Monoclonal Antibody (ICRF44), APC-eFluor 780, eBioscience™

货号：47-0118-41,47-0118-42

规格：25 Tests,100 Tests

价格：1416,2934

产品类型：抗体和ELISA

品牌：Thermo Fisher

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, APC-eFluor 780, eBioscience™
免疫原性：		用法：	5 μL(0.25 μg)/test(Flow)

Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.Applications Reported: This ICRF44 antibody has been reported for use in flow cytometric analysis.Applications Tested: This ICRF44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD11b Antibody (47-0118-42) in Flow Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (blue histogram) or Anti-Human CD11b APC-eFluor® 780 (purple histogram). Cells in thelymphocyte gate were used for analysis.

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参考文献：

1. Nature communicationsTumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis.2017.Authors Trabanelli S,Chevalier MF,Martinez-Usatorre A,Gomez-Cadena A,Salomé B,Lecciso M,Salvestrini V,Verdeil G,Racle J,Papayannidis C,Morita H,Pizzitola I,Grandclément C,Bohner P,Bruni E,Girotra M,Pallavi R,Falvo P,Leibundgut EO,Baerlocher GM,Carlo-Stella C,Taurino D,Santoro A,Spinelli O,Rambaldi A,Giarin E,Basso G,Tresoldi C,Ciceri F,Gfeller D,Akdis CA,Mazzarella L,Minucci S,Pelicci PG,Marcenaro E,McKenzie ANJ,Vanhecke D,Coukos G,Mavilio D,Curti A,Derré L,Jandus C2. Nature communicationsCD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.2016.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3. Nature communicationsdNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis....2015.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer4. Experimental hematologyInduction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacologic modulation....2015.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5. NatureReprogramming human endothelial cells to haematopoietic cells requires vascular induction.2014.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer

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