beta Tubulin Loading Control Monoclonal Antibody (BT7R), Biotin

货号：MA5-16308-BTIN

规格：50 µL

价格：2753

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：Biotin

类型:	单抗	同型对照：	IgG2a
免疫原性：	KLH conjugated peptide of the N-terminus of Beta-Tubulin.	用法：	1 µg/test(Flow);1:20-1:200(ICC);1:20-1:200(IF);1:10-1:500(IHC(P));1:500-1000(WB)

Product Specific InformationMA5-16308-BTIN has successfully been used for Western blot, IHC (P), FACS, ELISA and ICC/IFBackground/Target InformationBeta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

beta Tubulin Loading Control Antibody (MA5-16308-BTIN) in IHC (P)Immunohistochemistry analysis of Beta-Tubulin showing staining in the cytoskeleton of paraffin-embedded human lung adenocarcinoma (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Beta-Tubulin loading control antibody (Product # MA5-16308) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. beta Tubulin Loading Control Antibody (MA5-16308-BTIN) in WBWestern blot analysis of Beta-Tubulin was performed by loading 50 µg of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a biotinylated Beta-Tubulin monoclonal antibody (Product # MA5-16308-BTIN) at a dilution of 1:1000 for 1 hour at room temperature, followed by Streptavidin-HRP (Product # 21126) at a dilution of 1:20,000 for 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).

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