CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), FITC, eBioscience™

货号：11-3172-81,11-3172-82

规格：50 µg,100 µg

价格：957,2127

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

荧光染料：FITC

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, FITC, eBioscience™
免疫原性：		用法：	0.5 μg/test(Flow)

Description: The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naive mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.Applications Reported: This eBio927 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio927 antibody has been tested by flow cytometric analysis of SJL splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationBST2, also known as CD317, HM1.24 antigen, DAMP-2 and recently designated tetherin, is an integral membrane protein with a novel topology. The specific function of the BST2, encoded by the bone marrow stromal cell antigen 2 gene, is undetermined; however, it may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, NK cells and DCs (at protein level).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Staining of SJL splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2b kappa Isotype Control FITC (Product # 11-4031-82) (left) or 0.25 µg of Anti-Mouse CD317 (BST2, PDCA-1) FITC (right). Cells in the large forward scatter population were used for analysis.

参考文献：

1.Nature communications LAMTOR2 regulates dendritic cell homeostasis through FLT3-dependent mTOR signalling. "11-3172 was used in Flow cytometry/Cell sorting to define the effects from the conditional ablation of LAMTOR2 for the dendritic cell compartment in mice." AuthorsScheffler JM,Sparber F,Tripp CH,Herrmann C,Humenberger A,Blitz J,Romani N,Stoitzner P,Huber LA 2.Virology BST-2/tetherin is overexpressed in mammary gland and tumor tissues in MMTV-induced mammary cancer. "11-3172 was used in Flow cytometry/Cell sorting to suggest that overexpression of BST-2 in carcinoma tissues could be attributed to a factor that upregulates BST-2 upon oncogenesis initiation." AuthorsJones PH,Mahauad-Fernandez WD,Madison MN,Okeoma CM 3.Cytotechnology Changes in lamina propria dendritic cells on the oral administration of exogenous protein antigens during weaning. "11-3172 was used in Flow cytometry/Cell sorting to suggest that the behavior of tolerogenic PDCA-1(+) DCs may change at weaning with the removal of the immunoprotective components of maternal milk." AuthorsOhue R,Nakamoto M,Kitabatake N,Tani F 4.Retrovirology Bone marrow stromal cell antigen 2 (BST-2) restricts mouse mammary tumor virus (MMTV) replication in vivo. "11-3172 was used in Flow cytometry/Cell sorting to provide evidence that BST-2 restricts MMTV release from naturally infected cells and that BST-2 is an antiviral factor in vivo." AuthorsJones PH,Mehta HV,Maric M,Roller RJ,Okeoma CM 5.Journal of immunology (Baltimore, Md. : 1950) Increased hematopoietic cells in the mertk-/- mouse peritoneal cavity: a result of augmented migration. "11-3172 was used in Flow cytometry/Cell sorting to investigate whether Mertk regulates the composition of peritoneal cells that favour an autoimmune phenotype." AuthorsWilliams JC,Wagner NJ,Earp HS,Vilen BJ,Matsushima GK

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