CD11b Monoclonal Antibody (M1/70), FITC, eBioscience™

货号：11-0112-81,11-0112-82,11-0112-85,11-0112-86

规格：50 µg,100 µg,500 µg,1 mg

价格：749,1251,3022,5245

产品类型：流式抗体

品牌：eBioscience

抗原：CD11b

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：M1/70

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.5 µg/test (Flow）

产品详细信息Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: The M1/70 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1/70 antibody is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

数据

CD11b Antibody (11-0112-81) in FlowStaining of mouse bone marrow cells with 0.25 µg of Rat IgG2b K Isotype Control FITC(Product # 11-4031-82) (blue histogram) or 0.25 µg of Anti-Mouse CD11b FITC(purple histogram). Cells in the large scatter population were used for analysis. CD11b Antibody (11-0112-81)Journal of neuroinflammation 2018 -Fig. 2 Vascular congestion in IL-10 KO mice with malaria includes thrombi-containing monocytes and T cells. Immunofluorescent staining of fixed, frozen brain sections (30 mum) from P. chabaudi -infected IL-10 KO mice (day 8 p.i., n = 4 mice). a Confocal images (x 40) of IL-10 KO brain stained for fibrin (red). b Successive single-plane confocal images (x 40) of a 30-mum z-stack showing complete occlusion of a large vessel with residual fibrinogen (red). c Immunofluorescence staining of IL-10 KO brains showing fibrin staining of blood vessels (red) and leukocytes expressing CD45 (green, x 60) and d CD11b (green, x 40). e CTV + CD4 T cells (blue) from infected IL-10 KO mice were adoptively transferred into infection-matched IL-10 KO (day 7 p.i.) recipients 3.5 h before sacrifice. Frozen brain sections (day 7 p.i.) were stained for fibrin (red). Max intensity projection of a 30-mum z-stack (x 240) displayed from brain tissue of IL-10 KO mice co-stained with WT control samples ( n = 3-4 mice per group). Scale bars represent 30 mum ( a ), 50 mum ( b - d ), or 10 mum ( d ) CD11b Antibody (11-0112-81)eNeuro 2019 -Figure 6. Microglia expressing pro-/anti-inflammatory markers. Representative confocal immunofluorescent microscopy of CD11b (red) and pro-/anti-inflammatory markers (green) within the lumbar spinal cord are shown (images are from a WT B6 mouse on day 1 post-L5Tx surgery). Merged panels show colocalization of CD11b with the corresponding inflammatory marker. Scale bar: 40 mum.

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参考文献：

1.Frontiers in immunology ATP-Induced Inflammasome Activation and Pyroptosis Is Regulated by AMP-Activated Protein Kinase in Macrophages. 2.Frontiers in physiology Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung. 3.NPJ vaccines Neutrophil swarming and extracellular trap formation play a significant role in Alum adjuvant activity. 4.Frontiers in physiology Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung. 5.Aging and disease The Synergy of Aging and LPS Exposure in a Mouse Model of Parkinson's Disease.

技术参数

产品应用 Flow；IF；IHC；WB；IHC (F)

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