CD38 Monoclonal Antibody (90), Alexa Fluor 700

货号： 56-0381-82

规格：100μg

价格：2575

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：90

荧光染料：Alexa Fluor 700

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, Alexa Fluor 700, eBioscience™
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The 90 monoclonal antibody reacts with the mouse CD38 molecule, an ~42 kDa type II transmembrane protein. CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a role in lymphocyte activation and differentiation.Applications Reported: This 90 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 90 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD38 Antibody (56-0381-82) in Flow Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.06 µg of Rat IgG2a K Isotype Control Alexa Fluor® 700 (Product # 56-4321-80) (left) or 0.06 µg of Anti-Mouse CD38 Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Nature communicationsLimited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. The Journal of clinical investigationmTOR has distinct functions in generating versus sustaining humoral immunity."56038182 was used in flow cytometry to investigate the role of mTOR in plasma cell differentiation and antibody production in RAPTOR deficient cells."AuthorsJones DD,Gaudette BT,Wilmore JR,Chernova I,Bortnick A,Weiss BM,Allman D3. PloS oneBILL-cadherin/cadherin-17 contributes to the survival of memory B cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. mBioVirus-encoded microRNAs facilitate gammaherpesvirus latency and pathogenesis in vivo."56-0381 was used in Flow cytometry/Cell sorting to elucidate the contribution of gammaherpesvirus miRNAs to viral replication, survival, and pathogenesis in vivo."AuthorsFeldman ER,Kara M,Coleman CB,Grau KR,Oko LM,Krueger BJ,Renne R,van Dyk LF,Tibbetts SA5. PLoS neglected tropical diseasesInvestigating the role for IL-21 in rabies virus vaccine-induced immunity."56-0381 was used in Flow cytometry/Cell sorting to study the effects of knocking out IL-21 on the development of vaccine-induced immunity against rabies."AuthorsDorfmeier CL,Tzvetkov EP,Gatt A,McGettigan JP

技术参数

产品应用 Flow

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