CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700

货号：56-0341-82

规格：100μg

价格：3585

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：RAM34

荧光染料：Alexa Fluor 700

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, Alexa Fluor 700, eBioscience™
免疫原性：		用法：	1 µg/test(Flow）

Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Note: When staining with the RAM34 antibody an incubation time of 90 minutes is recommended to obtain the optimal signal to noise ratio.For more detailed information on staining with RAM34, please refer to Ema et al., listed under References.The Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD34 Antibody (56-0341-82) in Flow Staining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage eFluor® 450 Cocktail (Product # 88-7772-72),Anti-Mouse CD117 (c-Kit) APC (Product # 17-1171-82) and 1.0 µg of Anti-Mouse CD34 Alexa Fluor® 700.Lineage negative/low, viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14),were used for analysis.

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参考文献：

1. Cell stem cellAdipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration."56-0341 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone."AuthorsAmbrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ2. Cell stress and chaperonesRecombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells."56-0341 was used in Flow cytometry/Cell sorting to suggest that administration of exogenous HSP70 and application of mild heat stress may produce a "rejuvenating" effect on MSCs."AuthorsAndreeva NV,Zatsepina OG,Garbuz DG,Evgen'ev MB,Belyavsky AV3. PloS onehCD2-iCre and Vav-iCre mediated gene recombination patterns in murine hematopoietic cells."56-0341 was used in Flow cytometry/Cell sorting to assess the deletion of floxed genes during lymphocyte development, as mediated by the transgenes hCD2-iCre and Vav-iCre."AuthorsSiegemund S,Shepherd J,Xiao C,Sauer K4. Nature communicationsLECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells."56-0341 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which cytokines in the blood affect HSC homeostasis, showing that LECT2 drives HSC expansion and mobilisation."AuthorsLu XJ,Chen Q,Rong YJ,Yang GJ,Li CH,Xu NY,Yu CH,Wang HY,Zhang S,Shi YH,Chen J5. Stem cells (Dayton, Ohio)Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow

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