CD16/CD32 Monoclonal Antibody (93), FITC, eBioscience™

货号：11-0161-81,11-0161-82,11-0161-85

规格：50 µg,100 µg,500 µg

价格：851,1571,2920

产品类型：流式抗体

品牌：eBioscience

抗原：CD16/CD32

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：93

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.5 µg/test (Flow）

产品详细信息Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

数据

CD16/CD32 Antibody (11-0161-81) in FlowStaining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) PE(Product # 12-0454-82) and 0.25 µg of Anti-Mouse CD16/CD32 FITC. Total viablecells were used for analysis. CD16/CD32 Antibody (11-0161-81)mBio 2016 -FIG 2 Surface density and binding ability of Fcgamma receptors are unaltered by gliotoxin. (A to F) RAW 2.7 cells were treated with vehicle alone (DMSO) (left panels) or 500 ng/ml gliotoxin (right panels) for 30 min immediately before being labeled with either anti-FcgammaRI (alpha-FcgammaRI) (A and B) or anti-FcgammaRIIb/III (C and D) antibodies or exposed to Cy3-conjugated aggregated IgG (E and F). Surface expression of the indicated Fcgamma receptors (shown in green) was visualized by staining with Alexa Fluor 488-conjugated secondary antibodies, while nuclei were counterstained with DAPI (shown in blue). For each individual panel in panels A to F, channels are shown either individually as confocal XY sections (smaller images, left side), or merged as a Z-projection (larger image, right side). Bars = 10 um. alpha-rat IgG-488, anti-rat IgG conjugated to Alexa Fluor 488. (G and H) Flow cytometry histograms depicting the relative frequency of FcgammaRI-positive (G) and FcgammaRIIb/III-positive (H) RAW 264.7 cells under control (solid blue trace) and gliotoxin (solid red trace) conditions. Isotype traces, indicative of antibody-receptor interactions that occurred through the IgG2a Fc portion (as opposed to the variable region) are shown in dashed lines. The background signal (unlabeled macrophages) is depicted by the gray trace. (I) Quantification of the results shown in panels E and F. Relative fluorescence intensity of Cy3-conjugated aggregated IgG at the cell surface, repre

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参考文献：

1.Nature communicationsRational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.2.NatureMyeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.3.eLifelncRNA requirements for mouse acute myeloid leukemia and normal differentiation.4.Life sciencesRole of caveolin-3 in lymphocyte activation.5.Translational neurodegenerationCytometric profiling in various clinical forms of multiple sclerosis with respect to CD21+, CD32+, and CD35+ B and T cells.

技术参数

产品应用 Flow；IF

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