Mouse anti Human CD47 antibody, clone 1/1A4
货号：MCA2514A488T,MCA2514A488
规格：25 Tests/0.25ml,100 Tests/1ml
价格：1377,3213
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD47
物种：人
宿主：小鼠
抗体亚型：IgG2b
克隆号：1/1A4
荧光染料：Alexa Fluor 488
| 产品介绍 | | |
| Mouse anti Human CD47 antibody, clone 1/1A4recognizes human CD47, also known as integrin-associated protein, a 50kDa glycoprotein expressed on all haematopoietic cell lines, epithelial cells, endothelial cells, fibroblasts and many tumour cell lines.CD47 is involved in several cellular processes including regulation of T cell and dendritic cell activation, regulation of neutrophil (PMN) chemotaxis, caspase-independent apoptosis, and may act as a signal transducer in the regulation of cation fluxes across cell membranes. CD47 has been identified as a ligand for the inhibitory macrophage receptor known as signal regulatory protein alpha (SIRPalpha) and as a receptor for thrombospondin-1.Cross-linking of CD47 with clone 1/1A4 co-stimulates the proliferation of T cells (Waclaviceket al.1993). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Alexa Fluor 488 - liquid |
| Preparation | - MCA2514A488T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA2514A488: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
|
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Purified murine platelets |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Waclavicek, M.et al.(1997) T cell stimulation via CD47: agonistic and antagonistic effects of CD47 monoclonal antibody 1/1A4.J Immunol. 159 (11): 5345-54. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
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